A microtitration format has been configured by coating JEV antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to JEV forms a complex with the coated viral antigens. After washing away unbound material from the wells, an anti-porcine: horseradish peroxidase conjugate is added that binds to any porcine IgG antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, 3,3',5,5tetramethylbenzidine (TMB), are added to the wells.  Stop the reaction by adding stop solution. Within 15 minutes READ the absorbance at 450nm using 630nm as the reference wavelength if available.

Enzyme immunoassays for the qualitative of IgG antibodies against JEV in pig/sheep serum or plasma.

MATERIALS SUPPLIED

1  Microtiter Plate           1 pieces (96 wells)

2  Negative control       1 tube (0.7 ml)

3  Positive control           1 tube (0.7 ml)

4  Conjugate( anti-pig IgG )  1 bottle (11 ml)

5  Washing buffer 25×Concentrated   2 bottle (10 ml)

6  Substrate A 1 bottle (7 ml)

7  Substrate B 1 bottle (7 ml)

8  Stop solution 1 bottle (7 ml)

9  Sample diluent 2 bottle (25 ml)

10  Adhesive Foil  2